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. 2022 Mar 28;8:135. doi: 10.1038/s41420-022-00936-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A–E HCT-116 colon cancer cells were treated with VTD or DMSO for 48 h. Flow-cytometry experiments followed by FlowJo software analysis revealed that VTD significantly increases Annexin V-positive and Annexin V/PI-positive cell populations in a dose-dependent manner. F, G Graphical representation of flow-cytometry results in early and late apoptosis performed in triplicate and expressed as the mean ± standard deviation (**P < 0.01).