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. 2022 Mar 28;12:5238. doi: 10.1038/s41598-022-09191-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Chemometric analysis of metabolites among RHD and healthy controls. (a) Principal Component Analysis (PCA) score plot from RHD and healthy control. The green dots represent RHD patients and the red dots represent healthy controls in the 2D PCA score plots. (b) Partial least squares discriminant analysis (PLS-DA) score plot from RHD and healthy control. The two groups are well separated in the PLS-DA score plot, indicating that they had markedly different metabolic characteristics.