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. 2022 Mar 10;13:853449. doi: 10.3389/fpls.2022.853449

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Copyright © 2022 Khan, Zhang, Khan, Bi, Zhu, Luo and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Integral effects of biochar and nitrogen on glutamate synthase GS (A) SS and (B) FS in 2019–2020 and (C) SS and (D) FS in 2020–2021 and glutamine synthetaseGOGAT (E) SS and (F) FS in 2019–2020 and (G) SS and (H) FS in 2020–2021. SS: seedling stage, FS: flowering stage. B0: 0 t ha⁻¹biochar; B15: 15 t ha⁻¹biochar; B30: 30 t ha⁻¹biochar; B60: 60 t ha⁻¹biochar; N0: 0 kg ha⁻¹ nitrogen; N75: 75 kg ha⁻¹ nitrogen; N225: 225 kg ha⁻¹ nitrogen, and N450: 450 kg ha⁻¹ nitrogen, error bars represent standard error for each treatment in three replicates. Different letters on each bar represent significant differences according to the LSD test (p < 0.05).