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. 2022 Mar 28;13(3):272. doi: 10.1038/s41419-022-04620-3

Fig. 5. MIAT increases the expression of GPR158 by competitively binding to miR-613.

Fig. 5

A Venn diagram analysis of the predicted target mRNAs of miR-613 by starBase, miRDB, TargetScan, and mirDIP databases, and upregulated mRNAs in the GSE41445 dataset. B GPR158 expression in normal and osteosarcoma samples in the GSE41445 dataset. C Predicted binding sites between miR-613 and GPR158 by the starBase database. D Binding of miR-613 to GPR158 confirmed by dual-luciferase reporter assay in 293 T cells. E Expression of GPR158 determined by RT-qPCR in U2OS and MG63 cells transfected with miR-613 mimic or miR-613 inhibitor. F Western blot analysis of GPR158 protein in U2OS and MG63 cells transfected with miR-613 mimic or miR-613 inhibitor. G Expression of GPR158 determined by RT-qPCR in U2OS and MG63 cells transfected with oe-MIAT, miR-613 mimic, or both. H Western blot analysis of GPR158 protein in U2OS and MG63 cells transfected with oe-MIAT, miR-613 mimic, or both. *p < 0.05, **p < 0.01, ***p < 0.001. All experiments were conducted three times independently.