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. 2022 Mar 28;12:5264. doi: 10.1038/s41598-022-09183-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The metabolic activity (a) and the activity of alkaline phosphatase (ALP) (b) of hFOB 1.19 cells cultured on bare Ti6Al4V samples (Ti6Al4V), on Ti6Al4V samples coated with the O-terminated nanocrystalline diamond coating (O-NCD), or on Ti6Al4V samples coated with the O-terminated nanocrystalline diamond coating functionalized with BMP-7 (O-NCD + BMP-7) under differentiation conditions (a restrictive temperature of 39.5 °C) for 3 weeks. Mean ± S.E.M. * indicates a significant difference from the reference Ti6Al4V, while # shows a significant difference from the O-NCD coating; p < 0.05.