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. 2022 Mar 28;12:5264. doi: 10.1038/s41598-022-09183-z

Table 1.

Oligonucleotide primers used for qPCR amplification.

Gene Forward primer sequence Reverse primer sequence Product length (bp)
BGN 5′-CAG CCC GCC AAC TAG TCA-3' 5′-GGC CAG CAG AGA CAC GAG-3' 93
COL1A1 5′-CAG CCG CTT CAC CTA CAG C-3′ 5′-TTT TGT ATT CAA TCA CTG TCT TGC C-3′ 83
DCN 5′-GGA GAC TTT AAG AAC CTG AAG AAC C-3' 5′-CGT TCC AAC TTC ACC AAA GG-3' 104
GAPDH 5′-TGC ACC ACC AAC TGC TTA GC-3' 5′-GGC ATG GAC TGT GGT CAT GAG-3' 87
OSX 5′-GGC ACA AAG AAG CCG TAG TC-3' 5′-CAG GTG AAA GGA GCC CAT TA-3' 106
RUNX-2 5′-GCC TTC AAG GTG GTA GCC C-3' 5′-CGT TAC CCG CCA TGA CAG TA-3' 67
SPARC 5′-GTA CAT CGC CCT GGA TGA GT-3' 5′-CGA AGG GGA GGG TTA AAG AG-3' 124

BGN biglycan, COL1A1 collagen type I, DCN decorin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, OSX osterix, RUNX-2 runt-related transcription factor 2, SPARC osteonectin.