Table 1.
Gene | Forward primer sequence | Reverse primer sequence | Product length (bp) |
---|---|---|---|
BGN | 5′-CAG CCC GCC AAC TAG TCA-3' | 5′-GGC CAG CAG AGA CAC GAG-3' | 93 |
COL1A1 | 5′-CAG CCG CTT CAC CTA CAG C-3′ | 5′-TTT TGT ATT CAA TCA CTG TCT TGC C-3′ | 83 |
DCN | 5′-GGA GAC TTT AAG AAC CTG AAG AAC C-3' | 5′-CGT TCC AAC TTC ACC AAA GG-3' | 104 |
GAPDH | 5′-TGC ACC ACC AAC TGC TTA GC-3' | 5′-GGC ATG GAC TGT GGT CAT GAG-3' | 87 |
OSX | 5′-GGC ACA AAG AAG CCG TAG TC-3' | 5′-CAG GTG AAA GGA GCC CAT TA-3' | 106 |
RUNX-2 | 5′-GCC TTC AAG GTG GTA GCC C-3' | 5′-CGT TAC CCG CCA TGA CAG TA-3' | 67 |
SPARC | 5′-GTA CAT CGC CCT GGA TGA GT-3' | 5′-CGA AGG GGA GGG TTA AAG AG-3' | 124 |
BGN biglycan, COL1A1 collagen type I, DCN decorin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, OSX osterix, RUNX-2 runt-related transcription factor 2, SPARC osteonectin.