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. 2022 Mar 10;12:842356. doi: 10.3389/fonc.2022.842356

Figure 1.

Figure 1

Fbw7 impaired LDHA-mediated glucose metabolism reprogramming in ABC DLBCL. (A) QRT-PCR analysis of the relative mRNA expression in OCI-LY-3 and SU-DHL-2 cell lines. Cells were transfected with Flag-Fbw7 and vector control to detect Fbw7 expression. The mean ± SD is shown for three independent experiments. ***P < 0.001; one-way ANOVA. (B) Fbw7 inhibited intracellular LDH in ABC-DLBCL cells. The mean ± SD is shown for five independent experiments. ***P < 0.001; one-way ANOVA. (C) Fbw7 reduced intracellular lactate production in ABC-DLBCL cells. Results are presented as mean ± SD. ***P < 0.001; one-way ANOVA. (D) Fbw7 reduced ATP production. Results are presented as mean ± SD. ***P < 0.001; one-way ANOVA. (E) Western blotting results showed that overexpression of Fbw7 mainly inhibited LDHA expression in Glycolysis relative proteins. And the expression of Fbw7, LDHA, HK2, GLUT1, PDK1 were shown. (F) IHC staining results of Fbw7 and LDHA were shown with representative images from the same tumor case. The scale bar of “100x” indicates for 100μmand the scale bar of “200x” indicates for 50μm. (G) spearman correlation analysis between Fbw7 and LDHA in DLBCL FFPE tissues. Spearman rank correlation. P < 0.05 was considered to indicate a significant difference.