FIGURE 5.
Characterization and results of dynamic cultivation of endothelial colony forming cells (ECFCs), isolated from peripheral blood. (A) (Left side) ECFCs in I‐II with typical cobblestone morphology after up to 28 days, green immunofluorescence staining of vWF (III) and VE‐cadherin (IV), and capillary‐like structures in the angiogenesis assay (V), with typical antigen patterns in flow cytometry such as CD31+, CD146+, CD34+, CD133‐, CD45‐(right side). Scale of figures: I: 500 μm, II: 250 μm, III: 200 μm, IV: 100 μm, V: 1 mm. (B) Enforced cell orientation of ECFCs, detectable by staining of F‐actin filaments after pulsatile (1 Hz) cultivation with high shear stress (II) as compared to medium shear stress (I); ECFCs (MSS: medium shear stress = 5 dynes/cm2; HSS = high shear stress of 20 dynes/cm2; Scale in all images: 100 μm); adopted from [8]