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. 2022 Jan 20;18:17448069211060962. doi: 10.1177/17448069211060962

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — The expression of P2X7R have elevated in the CIBP group. (a) and (c) Co-immunostaining of P2X7R (green) and Iba-1 (microglial-specific marker, red) in the spinal dorsal horn of Sham rats and CIBP rats and the percentage of co-expressed in each cell type. ^**p < .01 versus Iba-1. (b) and (d) Representative immunofluorescence image and quantitative data for analysis showed the P2X7R intensity in the Sham group and CIBP group on POD 7, 14, 21. Data were presented as mean ± SD (n = 4 per group). ***p < .001 versus Sham group. (e) and (f) Representative Western blotting bands and quantitative data for analysis showed the protein expression levels of P2X7R in the Sham group and CIBP group on POD 7, 14, 21. β-actin was used as an internal control. Data were presented as mean ± SD (n = 4 per group). ***p < .001 versus Sham group.