Fig. 4.

. Effect of anti-CTLA-4 and anti-PD-1 immunotherapies in syngeneic intravenous based experimental metastasis models A-B. Representative pictures (A) and quantification of lung weight (B) after necropsy in the CT26 mouse colon cancer model. C, D. Representative pictures (C) and quantification of lung weight (D) after necropsy in the 4T1 mouse breast cancer model. Anti-CTLA-4 and anti-PD-1 were administered at days 6, 9, and 12 post-cell inoculation at respective doses of 100 and 200 µg i.p. per animal or with vehicle (Control). Statistical differences between the groups were determined using Kruskal-Wallis test followed by Dunn's multiple comparisons test (* p ≤ 0.05). Data represent mean and SD. N = 5–8 mice per group.