FIGURE 6.

HOTAIR-miR-152-CAMKIIα axis regulated osteoclast differentiation and apoptosis through NF-κB and MAPK/ERK 1/2 pathway (A) Expression of CAMKIIα mRNA in both miR-152 overexpressed (left) and HOTAIR-knockdown osteoclasts (right) was downregulated (B) Predicted binding sites for miR-152 in the 3′-UTR of CAMKIIα (C) RAW264.7 cells were transfected with the control construct (psiCHECK-2), or a construct encoding the wild-type CAMKIIα 3′-UTR, in addition to the miR-152 mimics (left) or miR-152 inhibitor (right). After 24 hs, luciferase activity in RAW264.7 lysates was detected (D) After transfection with HOTAIR siRNA or miR-152 mimics, the protein levels of CAMKIIα, Phosphorylated p65, p65, Phosphorylated ERK 1/2, and ERK 1/2 in mature osteoclasts were examined by western blotting. β-Tubulin was used to confirm equal protein loading (E) Effect of CAMKIIα on osteoclast differentiation-related gene expression in CAMKIIα siRNA-treated osteoclasts. Bars represent the mean ± SEM. *p < 0.05, **p < 0.01. The data are representative of three independent experiments.