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. 2022 Feb 18;18:26–41. doi: 10.1016/j.bioactmat.2022.02.012

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — In vitro biological properties of PLGA/Mg-GA MOF scaffolds. (A) Immunofluorescence images showing hBMSC morphology on different PLGA/Mg-GA MOF scaffolds. Human BMSC nuclei are stained with DAPI (blue) and the cytoskeleton stained with FITC (red). (B) A bespoke instrument was designed for seeding cells on scaffolds. (C) CCK-8 assay of hBMSCs on different scaffolds after 1, 3, 5, and 7 days. (D) Live/dead assay of hBMSCs cultured on scaffold surfaces for 3 days. Red staining indicates dead cells and green staining indicates live cells. (E) Osteogenic-related protein expression in hBMSCs on different scaffolds by western blotting. Data are presented as the mean ± standard deviation (SD) (n = 6, *P < 0.05, **P < 0.01, ***P < 0.001).