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. 2022 Mar 10;12:776996. doi: 10.3389/fcimb.2022.776996

Figure 3.

Figure 3

Transcriptional pattern of HUVECs in response to P. gingivalis infection. (A) Transcriptional pattern of HUVECs infected with P. gingivalis (infection) and uninfected cells (control) showing clear divergence as determined via PCA. (B) Volcano plot of differentially expressed genes in HUVECs. (C) KEGG analysis of upregulated genes in HUVECs during infection. (D) KEGG analysis of downregulated genes in HUVECs during infection. (E) Validation of RNA sequencing data via qRT-PCR. The expression of MTCO2, MTCO3, UQCRB, and NDUFB10 in endothelial cells. FC of the expression of each gene in HUVECs infected with P. gingivalis relative to that corresponding to uninfected cells. HUVECs, human umbilical vein endothelial cells; PCA, principal component analysis; FC, fold change; KEGG, Kyoto Encyclopedia of Genes and Genomes. The results are the average of three independent experiments and are presented as mean ± SD. **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.