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. 2022 Mar 10;13:856914. doi: 10.3389/fphar.2022.856914

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Copyright © 2022 Liu, Cheng, Zhang, Shao, Chen, Han, Zhou and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative images of immunohistochemistry showing CD11b-immunoreactive microglia (A) and quantitative comparison of total (B) and activated (C) microglia in the PVN in rats fed a normal salt (NS) diet or a high salt (HS) diet and simultaneously treated with vehicle (VEH) or 1.0% 3,3-Dimethyl-1-butanol (DMB, an inhibitor of trimethylamine formation). Scale bar = 100 μm. All data are expressed as mean ± SEM (n = 5 rats/per group). ∗p < 0.05 vs. NS + VEH; †p < 0.05, HS + DMB vs. HS + VEH.