FIGURE 6.

Representative images of immunohistochemistry showing Fra-LI immunoreactivity (A), a marker of neuronal excitation and quantification of Fra-LI positive neurons in the PVN (B), and plasma levels of norepinephrine (C), a marker of sympathetic nerve activity, in rats fed a normal salt (NS) diet or a high salt (HS) diet and simultaneously treated with vehicle (VEH) or 1.0% 3,3-Dimethyl-1-butanol (DMB, an inhibitor of trimethylamine formation). Dark dots in A indicate single activated neurons. Scale bar = 100 μm. All data are expressed as mean ± SEM (n = 5-8 rats/per group). ∗p < 0.05 vs. NS + VEH; †p < 0.05, HS + DMB vs. HS + VEH.