TABLE 1.
Gene | Primers | Sequences |
---|---|---|
TNF-α | Forward primer | 5′- AAATGGGCTCCCTCTCATCAGTTC -3′ |
Reverse primer | 5′- TCTGCTTGGTGGTTTGCTACGAC -3′ | |
IL-1β | Forward primer | 5′- CACCTCTCAAGCAGAGCACAG -3′ |
Reverse primer | 5′- GGGTTCCATGGTGAAGTCAAC -3′ | |
IL-6 | Forward primer | 5′- TCCTACCCCAACTTCCAATGCTC -3′ |
Reverse primer | 5′- TTGGATGGTCTTGGTCCTTAGCC -3′ | |
CD11b | Forward primer | 5′- TGACGGCTCCGGTAGCAT -3′ |
Reverse primer | 5′- CCATCACAGTTGAGACAAATTCCT-3′ | |
GFAP | Forward primer | 5′- GGGCGAAGAAAACCGCAT -3′ |
Reverse primer | 5′- TCTGGAGGTTGGAGAAAGTCTGT -3′ | |
NOX2 | Forward primer | 5′- CTGCCAGTGTGTCGGAATCT -3′ |
Reverse primer | 5′- TGTGAATGGCCGTGTGAAGT -3′ | |
NOX4 | Forward primer | 5′- GGATCACAGAAGGTCCCTAGC -3′ |
Reverse primer | 5′- AGAAGTTCAGGGCGTTCACC -3′ | |
β-actin | Forward primer | 5′- TGAAGATCAAGATCATTGCTCCTC -3′ |
Reverse primer | 5′- AGCCACCAATCCACACAGAGT -3′ |
TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; CD11b, cluster of differentiation molecule 11b; GFAP, glial fibrillary acidic protein; NOX2, NADPH oxidase 2.