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. 2022 Mar 10;13:856914. doi: 10.3389/fphar.2022.856914

TABLE 1.

Sequences for primers.

Gene Primers Sequences
TNF-α Forward primer 5′- AAA​TGG​GCT​CCC​TCT​CAT​CAG​TTC -3′
Reverse primer 5′- TCT​GCT​TGG​TGG​TTT​GCT​ACG​AC -3′
IL-1β Forward primer 5′- CAC​CTC​TCA​AGC​AGA​GCA​CAG -3′
Reverse primer 5′- GGG​TTC​CAT​GGT​GAA​GTC​AAC -3′
IL-6 Forward primer 5′- TCC​TAC​CCC​AAC​TTC​CAA​TGC​TC -3′
Reverse primer 5′- TTG​GAT​GGT​CTT​GGT​CCT​TAG​CC -3′
CD11b Forward primer 5′- TGACGGCTCCGGTAGCAT -3′
Reverse primer 5′- CCA​TCA​CAG​TTG​AGA​CAA​ATT​CCT-3′
GFAP Forward primer 5′- GGGCGAAGAAAACCGCAT -3′
Reverse primer 5′- TCT​GGA​GGT​TGG​AGA​AAG​TCT​GT -3′
NOX2 Forward primer 5′- CTG​CCA​GTG​TGT​CGG​AAT​CT -3′
Reverse primer 5′- TGT​GAA​TGG​CCG​TGT​GAA​GT -3′
NOX4 Forward primer 5′- GGA​TCA​CAG​AAG​GTC​CCT​AGC -3′
Reverse primer 5′- AGA​AGT​TCA​GGG​CGT​TCA​CC -3′
β-actin Forward primer 5′- TGA​AGA​TCA​AGA​TCA​TTG​CTC​CTC -3′
Reverse primer 5′- AGC​CAC​CAA​TCC​ACA​CAG​AGT -3′

TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; CD11b, cluster of differentiation molecule 11b; GFAP, glial fibrillary acidic protein; NOX2, NADPH oxidase 2.