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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: J Am Chem Soc. 2021 Nov 1;143(44):18454–18466. doi: 10.1021/jacs.1c06680

Figure 5:

Figure 5:

AMUPol is deactivated by cellular biomass. (A) DNP enhancements decrease and (B) TB,on values from saturation recovery experiments for protein increase with longer room temperature exposure to AMUPol to cellular biomass before freezing. Lysed cells with 10 mM AMUPol (light green open circles) were flash frozen after indicated room temperature incubation times. Intact cells where the 10 mM AMUPol was introduced by incubation (dark green closed circles) and where the 10 mM AMUPol was introduced by electroporation (dark green open circles) require room temperature manipulation before reducing the temperature at a rate of 1 °C/min. An additional 25 minutes (gray bar) was added to the room temperature incubation time for intact cells to visually represent this difference in sample preparation. Analyses for additional biomass components are presented in Supplemental Figure S5.