Fig. 3.

Analysis of the defatting effect and DNA content of ANXs. (A) Oil red O staining of ANXs and Natural SN (the picture below is an enlarged view of the yellow area in the picture above). (B, C, D) Chromatogram analysis of 37 fatty acids of the natural SN, CD NG and CD + scCO₂ NG (each peak represents one of the fatty acids). (E) The relationship between fat content and extraction cycle. (F) The fat content of ANXs and Natural SN. Data are presented as the mean ± SD (n = 3). (G) The percent decrease of 19 fatty acids with the highest content among the 37 fatty acids in the two kinds of ANXs. Data are presented as the mean ± SD (n = 3). (H) DNA content of ANXs and Natural SN. Data are presented as the mean ± SD (n = 5). Statistical analysis: n.s. no significances, **p < 0.01, *p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)