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Protein and mRNA expression levels of EMT associated markers in the miR-182-5p transfected PC-3 cells. (a) Expression analysis of EMT markers (β-Catenin, E-Cadherin, N-Cadherin, Vimentin, and ZEB1) by Western blot and protein levels’ quantification (Image J). (b) p-AKT expression analysis by Western blot and expression levels’ quantification (Image J). GAPDH was used as loading reference. NC = negative control; I = miR-182-5p-inh; M = miR-182-5p-mimic.
