Figure 4.

Maturation and toxicity on D1 cells induced by the active product purified from the extract of C. lepadiformis sp. B. (A) Expression of cell surface markers of D1 cells after treatment with the active molecule from 1 to 10 µg/mL (n = 3). Data are expressed as Mean Fluorescence Intensity (MFI) of CD80, CD40 and MHC-II compared to cells treated only with vehicle (dashed lines) after 24 h; (B) Cell viability expressed as percentage of cytotoxicity after stimulation of D1 cells with the active molecule in the same range of concentrations. A nonlinear regression analysis was performed for the estimation of the IC₅₀ value as plotted in the figure.