Figure 1.

Modest changes in inflammatory cytokine profiling of sera from Gata1^low mice with age and with respect to CD1 littermates wild-type at the Gata1 locus and to Gata1^lowPsel^null mice. (A) Heatmap of detectable cytokines. (B) Concentration values for each cytokine detected. P values were calculated with multiple comparison t-tests for each cytokine. * p < 0.05, *** p < 0.01, **** p < 0.0001. (C) Serum levels determined by ELISA of TGF-β1 (left), CXCL1 (middle), and LCN2 (right) in CD1 mice wild-type at the Gata1 locus, in Gata1^low mice, and in mice lacking the Psel gene with and without the Gata1^low mutation. The number of mice analyzed in each experimental group is indicated by n. Statistical analyses were performed with multiple comparison test. Since age and sex analyzed as independent variables did not reveal statistically significant differences in most of the groups (see Figure S3 and Table S1, Supplementary Materials), results obtained at different ages in females and males were pooled. The number of mice analyzed in each group was TGF-β1: adult CD1 males n = 5; adult CD1 females n = 3, old CD1 males n = 4; old CD1 females = 3, total CD1 n = 15; adult Psel^null males: n = 3; adult Psel^null females n = 3, total Psel^null n = 6; old Gata1^lowPsel^null females n = 3, total Gata1^lowPsel^null = 3; LCN2: adult CD1 males n = 5; adult CD1 females = 4, old CD1 males n = 5; old CD1 females n = 5, total CD1 n = 19; old Gata1^low males n = 3, old Gata1^low females n = 3, total Gata1^low n = 6; old Psel^null females n = 3, total Psel^null n = 3; old Gata1^lowPsel^null males n = 3, old Gata1^lowPsel^null females n = 3, total Gata1^lowPsel^null n = 6; CXCL1: adult CD1 males n = 8; adult CD1 females n = 6, old CD1 males n = 6; old CD1 females = 5, total CD1 n = 25; adult Gata1^low males n = 3, adult Gata1^low females n = 3, old Gata1^low males n = 4, old Gata1^low females n = 5, total Gata1^low n = 15; adult Psel^null males: n = 5; adult Psel^null females n = 3, old Psel^null females n = 3, total Psel^null n = 11; adult Gata1^lowPsel^null males n = 3, old Gata1^lowPsel^null males n = 3, old Gata1^lowPsel^null females n = 3, total Gata1^lowPsel^null n = 9. Abbreviations: TGF-β1, transforming growth factor β1; LCN2, lipocalin-2; Psel, P-selectin; IL6, IL1a, IL5, IL12p70, IL12p40, IL13, and IL9, interleukin 6, 1a, 5, 12p70 and p40, 13 and 9, respectively; LIX, also known as CXCL5 (C-X-C motif chemokine 5); G-CSF, granulocyte-colony stimulating factor; KC, also known as CXCL1 (C-X-C motif chemokine 1); MCP1, monocyte chemoattractant 1; MIP1a and MIP1b, macrophage inflammatory protein a and b; MIG, also known as CXCL9; TNFa, tumor necrosis factors a.