Figure 4.
The bone marrow from Gata1low mice (12–14 months of age) contains levels of endothelial cells, mesenchymal stem cells, osteoblasts, and megakaryocytes greater than those present in the bone marrow from wild-type littermates. The levels of these cells in the mutant mice were greatly reduced by treatment with a TGF-β1 inhibitor and/or by deletion of the P-sel gene. Cells were identified according to previously described flow cytometry criteria [34,35] (see Figure S1 for the detail of the gates, Supplementary Materials). Results are presented as mean (±SD) and as values of individual mice (each dot an individual mouse). P values were calculated with Tukey multiple comparison and those statistically significant are indicated in the panels. Abbreviations: ECs, endothelial cells; MSCs, mesenchymal stem cells; OBCs, osteoblasts; MKs, megakaryocytes; Neu, neutrophils; WT, wild-type; Psel, Pselectin; TGF-β inh, transforming growth factors β inhibitor.