Figure 1.

ScRNA-seq reveals multiple cell types in preovulatory follicles undergoing IVF treatment. (A) Schematic representation of a preovulatory follicle and the experimental procedure of the scRNA-seq study. (B) UMAP plot where cells that share similar transcriptome profiles are grouped by colours representing unsupervised clustering results. (C) Violin plots showing the expression of representative differential genes for each cluster. (D) UMAP plot of 14,592 high-quality cells from all samples to visualize the clustering of six cell types identified according to the expression of known marker genes, including granulosa cells (GCs), macrophages, dendritic cells (DCs), T cells, epithelial cells, and neutrophils. (E) Heat map showing the expression of established marker genes for each cell type in (D). (F) UMAP plots showing the expression patterns of characteristic genes in six cell types. Cells in the plots are coloured with the expression levels of the indicated genes, with a gradient from grey to red indicating the lowest to the highest gene expression level.