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. 2022 Jan 18;12(2):157. doi: 10.3390/biom12020157

Figure 4.

Figure 4

Compounds inhibit the aggregation of ARQ46; ThT assay with L-ARQ46 was performed at 37 °C in TBS pH 7.5. The progression of fluorescence intensity was measured every 30 min at λex = 410 nm and λem = 482 nm with 30 s agitation at 300 rpm before every measurement. (A) Fluorescence intensity measured at 90 h of the aggregation assay starting with 15 µM monomeric ARQ46 without peptide and with 15 µM QF2D-1, QF2D-2, QF2D-3, QF2D-4, QF2D-5, QF2D-6, QF2D-7, QF2D-8 or QF2D-9. (B) Lag time estimated from the aggregation assay of 15 µM monomeric ARQ46 without peptide and with 15 µM QF2D-1, QF2D-2, QF2D-3, QF2D-4, QF2D-5, QF2D-6, QF2D-7, QF2D-8 or QF2D-9. (C) Measurement example of the described ThT-assay with ARQ46 (dark grey) and QF2D-2 (black). QF2D-2 in TBS buffer alone served as a control (light grey). The mean fluorescence intensity is shown for each time point. The experiment was performed in three-fold determination.