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. 2022 Jan 18;12(2):157. doi: 10.3390/biom12020157

Figure 6.

Figure 6

Investigation of structural change of ARQ46 in presence of QF2D-1 or QF2D-2 with CD spectroscopy; ARQ46 was disaggregated with 1:1 TFA/HFIP and diluted to 50 µM in TBS pH 7.5. 50 µM QF2D-1 or QF2D-2 were added to one sample. As a control and reference, 50 µM QF2D-1 or QF2D-2 alone were measured. (A) Spectra measured directly after disaggregation for 50 µM ARQ46 (rose, dashed)- referenced with buffer; 50 µM ARQ46 after 24 h incubation at 37 °C (rose, straight) and 50 µM ARQ46 after 48 h incubation at 37 °C (red, straight) (B) Spectra measured directly after disaggregation for 50 µM ARQ46 (rose, dashed)- referenced with buffer; equimolar ARQ46 and QF2D-1 (light purple, dashed)- referenced with QF2D-1 in TBS; 50 µM ARQ46 after 24 h incubation at 37 °C (rose, straight) and equimolar ARQ46 and QF2D-1 (light purple, straight)- referenced with QF2D-1 in TBS and 50 µM ARQ46 after 48 h incubation at 37 °C (red, straight) and equimolar ARQ46 and QF2D-1 (purple, straight)- referenced with QF2D-1 in TBS (C) Spectra measured directly after disaggregation for 50 µM ARQ46 (rose, dashed)- referenced with buffer and equimolar ARQ46 and QF2D-2 (light blue, dashed)- referenced with QF2D-2 in TBS; 50 µM ARQ46 after 24 h incubation at 37 °C (rose, straight) and equimolar ARQ46 and QF2D-2 (light blue, straight)- referenced with QF2D-2 in TBS and 50 µM ARQ46 after 48 h incubation at 37 °C (red, straight) and equimolar ARQ46 and QF2D-2 (light blue, straight)- referenced with QF2D-2 in TBS (D) Spectra measured directly after disaggregation for 50 µM ARQ46 (rose, dashed)- referenced with buffer; equimolar ARQ46 and QF2D-2 (light blue, dashed)- referenced with QF2D-2 in TBS; 50 µM ARQ46 after 24 h incubation at 37 °C (rose, straight) and equimolar ARQ46 and QF2D-2 (light blue, straight)- referenced with QF2D-2 in TBS; 50 µM ARQ46 after 48 h incubation at 37 °C (red) and equimolar ARQ46 and QF2D-2 (blue)- referenced with QF2D-2 in TBS; 50 µM ARQ46 after 168 h incubation at 37 °C (dark red) and equimolar ARQ46 and QF2D-2 (dark blue)- referenced with QF2D-2 in TBS.