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. 2022 Feb 3;12(2):247. doi: 10.3390/biom12020247

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Nitric oxide synthases, nitrotyrosine, and inflammation marker expression in granulocytes and IL10 in the plasma of patients with chronic myeloid leukemia (CML). (a) Inducible nitric oxide synthase (iNOS), endothelial NOS (eNOS), and nitrotyrosine levels in the granulocytes of patients with CML (n = 13) and healthy subjects (Control, n = 6). In total, 700 cells per cytospin were counted. (b) Frequency of NFkB, IL-6, and active/total p53 ratio in granulocytes and the IL10 level in the plasma of patients with CML (n = 13) and healthy subjects (Control, n = 6). In total, 800 cells per cytospin were counted using a computer supported imaging system (Analysis Pro 3.1) connected to a light microscope with an objective magnification of 40. Below the graphs, sections of cytospins from patients and healthy controls after immunocytochemical staining are shown (40× objective magnification). Values are mean ± SEM. ** p < 0.01; and *** p < 0.001 vs. control.