Table 2.
Refinement statistics after restrained and His-unrestrained refinement of the seven structures in this study.
| Crystallization Condition | No. of Mols/Asymmetric Unit | Resolution (Å) | Restrained Refinement | Histidine-Unrestrained Refinement | |||
|---|---|---|---|---|---|---|---|
| Final RWork/RFree [%] | Final RWork/RFree [%] | Final RMSD Bond lengths [Å] | Final RMSD Bond Angles [°] | ||||
| LsAA9A_Ec_1 | 0.1 M citric acid pH 3.5 and 3.0 M NaCl | 1 | 1.09 | 11.04/12.36 | 11.39/12.82 | 0.0131 | 1.8818 |
| LsAA9A_Ec_2 | 0.1 M citric acid pH 3.5 and 3.0 M NaCl | 1 | 1.09 | 12.16/13.79 | 12.62/13.93 | 0.0170 | 1.9397 |
| TaAA9A | 0.2 M MgCl2, 0.1 M HEPES pH 7.5, 22% polyacrylic acid | 2 | 1.06 | 11.94/14.17 | 11.80/12.70 | 0.0121 | 2.0481 |
| 5O2X | 1.6 M ammonium sulfate, 0.1 M citric acid 4.0 | 1 | 0.95 | 11.29/12.53 | 11.39/12.58 | 0.0139 | 1.9586 |
| 4EIR | 20% PEG 3350, pH 6.7 | 2 | 1.10 | 12.07/13.52 | 11.20/12.73 | 0.0147 | 1.8145 |
| 5OPF | 0.04 M potassium phosphate monobasic, 16% w/v PEG8000, 20% v/v glycerol | 1 | 1.08 | 11.49/13.86 | 11.79/14.02 | 0.0124 | 1.7270 |
| 4QI8 | 0.2M ammonium nitrate, 20% (w/v) PEG 3350, pH 7.0 | 2 | 1.10 | 11.98/14.60 | 12.08/14.62 | 0.0161 | 1.8468 |