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. 2022 Feb 1;12(2):239. doi: 10.3390/biom12020239

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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ErbB2 expression is modulated by M2 agonist APE. (A) qRT-PCR analysis shows the decreased expression of erbB2 receptor transcripts after 16 h of APE treatment (APE 16 h: *** p < 0.001; n = 3); (B) Representative Western blotting showing the decreased expression of erbB2 receptor after 16 and 24 h of APE treatment; the graph represents the average of the OD ± SEM of the bands normalized against the reference protein (**** p < 0.0001; n = 3). Calreticulin was used as reference protein considering the prevalent accumulation of erbB2 receptors in endoplasmic reticulum (ER) after APE treatment.