Figure 1.

Effects of icv SPX administration on food intake, body weight and POMC and AgRP mRNA levels. Eight-week-old male mice were icv-injected with saline or SPX (10 μg). Food intake (A) and body weight gain (B) were measured for 24 h after injection. Body temperature (C) and locomotor activity (D) were observed for 48 h using a vital-view telemetry system. SPX was injected at the beginning of light period as indicated with arrows. Shaded areas represent the dark period. No difference was observed between injection with saline and SPX (n = 4/group). POMC (E) and AgRP (F) mRNA levels were assessed by qRT-PCR analysis. Data are presented as mean ± S.E.M. *, p < 0.05; ***, p < 0.001.