Figure 3.

Effect of SPX synthesis blockade on the anorexigenic effects of leptin. To inhibit SPX synthesis in the brain, eight-week-old male mice were icv-injected with 2 μg of antisense (AS) or scrambled (SCR) oligodeoxynucleotide (ODN) once a day for 2 days. (A) MBH samples were collected at 24 h after the second ODN injection and SPX mRNA levels were determined using qRT-PCR. (B,C) Mice injected twice with AS ODN were fasted for 24 h before icv leptin (2.5 μg) injection. Food intake (B) and body weight gain (C) were measured for 24 h after leptin injection. Data are presented as mean ± S.E.M. *, p < 0.05; **, p < 0.01.