Figure 5.

Anorexigenic effect of leptin-induced SPX via GALR2. (A,B) Eight-week-old male mice were fasted for 24 h and icv-injected with M871 (GALR2 antagonist, 5 nM) or SNAP (GALR3 antagonist, 5 nM) 1 h before icv leptin administration. Food intake (A) and body weight gain (B) were measured for 24 h after leptin injection. (C) Luciferase reporter constructs containing the 5′-flanking region of POMC (300 ng) were transfected into mHypoA cells. After 24 h, cells were pre-treated with M871 (1 μM) or SNAP (1 μM) at 30 min before SPX (300 ng/mL) treatment. Then, cells were harvested for luciferase and β-galactosidase assays at 1 h after SPX treatment. Data are presented as mean ± S.E.M. *, p < 0.05; **, p < 0.01; ***, p < 0.001.