Fig. 5. Treatment with inhaled RDV following SARS-CoV-2 infection reduces viral load in respiratory tract tissues.

AGM were inoculated with SARS-CoV-2 and treated with inhaled (blue symbols) or IV (red symbols) RDV (closed symbols) or vehicle (open symbols) beginning at 8 hours post-infection (n=6 each). Respiratory tissues were collected 6 days post-inoculation. SARS-CoV-2 (A) genomic and (B) subgenomic RNA copies were quantified by RT-qPCR. (C) Infectious SARS-CoV-2 titers were determined by a TCID₅₀ assay. Samples that were below the lower limit of quantification (LLOQ, dotted lines) for the assay were assigned a value equal to the LLOQ for analyses. Horizontal bars indicate median values. *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001; data were analyzed by a repeated measures two-way ANOVA with Bonferroni post-hoc correction.