Figure 5.

Fluorescence lifetime imaging in NSCLC tissue using FAP3 probe and a clinically approved FLIM system. (A) Representative images showing the change in fluorescence intensity and lifetime after 488nm excitation of a NSCLC tumor tissue sample over time when incubated with FAP3 at 5µM, with lowest panel demonstrating the late time point with presence of the inhibitor Talabostat; (B) Representative plots showing the change in intensity and fluorescence lifetime over 40 mins on a NSCLC tissue sample; (C) Aggregate analysis across 5 NSCLC samples at 40 minutes demonstrating a significant increase in fluorescence lifetime and intensity, with inhibition in the presence of Talabostat. N=5, analysis by paired t-test, *p < 0.05, **p < 0.01.