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. Author manuscript; available in PMC: 2022 Mar 29.
Published in final edited form as: J Mol Cell Cardiol. 2018 Nov 15;126:36–49. doi: 10.1016/j.yjmcc.2018.11.008

Fig. 5. Phosphorylation of Cx43 residue Y313 contributes to reduced gap junction intercellular communication (GJIC).

Fig. 5.

The level of GJIC in HeLa cells stably expressing Cx43 WT or phosphorylation eliminating mutants (labeled above) transiently transfected with v-Src was determined using the scrape loading transfer assay. A) Representative immunofluorescence images of HeLa cells stably expressing Cx43 WT or phosphorylation eliminating mutants (labeled above) transiently transfected with v-Src (Lucifer yellow, green; DAPI, Blue). B) Quantification of the distance of Lucifer Yellow transfer shows the effect of inhibiting v-Src phosphorylation of different Cx43 tyrosine residues. Results presented as the mean + s.e.m. (n = 3). Statistics used to analyze the data was one-way ANOVA with a Newman-Keuls post-hoc analysis (n = 3, *P < .05, ***P < .001).