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. Author manuscript; available in PMC: 2022 Mar 29.
Published in final edited form as: J Mol Cell Cardiol. 2018 Nov 15;126:36–49. doi: 10.1016/j.yjmcc.2018.11.008

Fig. 9. Phosphorylation of Cx43 residue Y313 is increased in cardiac left ventricle hypertrophy (LVH) samples.

Fig. 9.

Paired (A) Cx43 and (B) Cx43 pY313 immunohistochemistry staining in normal and LVH samples cut in transverse section. Green and red arrows indicate staining for total Cx43 and Cx43 pY313, respectively. Boxplots depicting distribution of (C) Cx43 and (D) Cx43 pY313 staining scores when combining each sample in normal (n = 2), LVH without dilatation (n = 11), and LVH with dilatation (n = 11) groups. The field of view for each sample was 40×. E) Cx43 pY313 staining in LVH samples cut in the longitudinal section left - LVH without dilation, right LVH with dilation. Red arrows indicate staining of Cx43 pY313 at the intercalated disc. Green arrows indicate intracellular staining of Cx43 pY313. F) Cx43 pY313 antibody was pre-incubated with excess phospho-peptide 12 h before being applied to the human tissue array slide. The same immunohistochemistry staining protocol was used as in (A) and (B).