Table I.
Phospho-Tyr containing peptides identified from mass spectrometry of the Cx43CT in vitro phosphorylated by Src.
| Peptide sequence | Start-end (residue number) | Average mascot Delta ion score | aNumber of phospho-peptides | Phosphorylation site |
|---|---|---|---|---|
|
| ||||
| GRSDPyHATTGPLSPSK | 242–258 | 16.9 | 4 | Y247 |
| GRSDPyHATTGPLSPSKDCGSPK | 242–264 | 17.0 | 1 | Y247 |
| SDPyHATTGPLSPSK | 244–258 | 19.7 | 8 | Y247 |
| SDPyHATTGPLSPSKDCGSPK | 244–264 | 12.8 | 2 | Y247 |
| yAYFNGCSSPTAPLSPMSPPGYK | 265–287 | 10.8 | 10 | Y265 |
| DRGSPTyAYFNGCSSPTAPLSPMSPPGYK | 259–287 | 11.8 | 2 | Y265 |
| YAyFNGCSSPTAPLSPMSPPGYKLVTGDR | 265–293 | 8.0 | 2 | Y267 |
| yAyFNGCSSPTAPLSPMSPPGYK | 265–287 | 26.9 | 10 | Y265, Y267 |
| QASEQNWANySAEQNR | 304–319 | 16.9 | 8 | Y313 |
a
Peptides with Mascot Delta Ion Score > 7 were used to obtain the average Mascot Delta Ion Score.