TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH10B | F′ mcrA Δ(mrr-hsdRMS-mrcBC) Φ80ΔlacZDM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu)7697 galU galK1 rpsL nupG | Gibco BRL |
| E. coli JM109 | endA1 hsdR17 gyrA96 Δ(lac proA) recA1 relA supE44 thi F′ (lacIqlacZΔM15 proAB+ traD36) | 48 |
| Rifampin-resistant E. coli JM109 obtained in vitro | Rifampin-resistant | This study |
| C. meningosepticum PINT | Extended-spectrum cephalosporin and carbapenem resistant | This study |
| C. meningosepticum AMA | Extended-spectrum cephalosporin and carbapenem resistant | This study |
| C. meningosepticum GEO | Extended-spectrum cephalosporin and carbapenem resistant | This study |
| C. meningosepticum CIP 6058 | Extended-spectrum cephalosporin and carbapenem resistant | IPa |
| C. meningosepticum CIP 6059 | Extended-spectrum cephalosporin and carbapenem resistant | IP |
| C. meningosepticum CIP 7830 | Extended-spectrum cephalosporin and carbapenem resistant | IP |
| C. meningosepticum CIP 7905 | Extended-spectrum cephalosporin and carbapenem resistant | IP |
| C. meningosepticum AB1572 | Extended-spectrum cephalosporin and carbapenem resistant | 7 |
| C. meningosepticum H01, J100 | Extended-spectrum cephalosporin and carbapenem resistant | 7 |
| C. indologenes CIP 101026 | Carbapenem resistant | IP |
| C. indologenes PIT | Carbapenem resistant | This study |
| S. multivorum CIP 103686 | Carbapenem resistant | IP |
| M. odoratus CIP 103105 | Carbapenem resistant | IP |
| Plasmids | ||
| pBK-CMV phagemid | Neomycin and kanamycin resistant | Stratagene |
| pBR322 | Recombinant plasmid containing the 560-bp SspI-PstI internal fragment of blaTEM-1 | 42 |
| pHUC37 | Recombinant plasmid containing the 435-bp PstI-NotI internal fragment of blaSHV-3 | 24 |
| pPZ1 | Recombinant plasmid containing the 1.1-kb SnaBI fragment of blaPER-1 | 25 |
| pRLT1 | Recombinant plasmid containing the 329-bp DraI-XmnI internal fragment of blaVEB-1 | 32 |
| pPL1 | Recombinant plasmid containing the 396-bp EcoRI-PvuI internal fragment of blaOXA-18 | 29 |
| pBS1 | 1.9-kb Sau3AI fragment from C. meningosepticum PINT cloned into pBK-CMV | This study |
a
IP, Institut Pasteur, Paris, France.