Fig. 4.

The microbiota of humanized responder mice is different from that of non-responders. 16S rDNA sequencing was performed on the feces collected from the anti-PD-1-treated pooled microbiota mice 2 weeks post-colonization and prior to the initial treatment, as well as individual patient inoculum microbiota and their associated pools. A Principal coordinates analysis (PCoA) showing beta diversity measured by weighted UniFrac distance between individual human donors (R: n = 4; NR: n = 6), pooled inoculums (R: n = 1; NR: n = 1) and mouse feces 2 weeks post-colonization with pooled donor inoculums and at endpoint (R: 2 weeks n = 8, endpoint n = 9; NR: 2 weeks n = 4, endpoint n = 8). B Principal coordinates analysis (PCoA) showing beta diversity measured by weighted UniFrac distance between individual mouse feces 2 weeks post-colonization with pooled donor inoculums (R: n = 8; NR: n = 4) lme P = 0.001. C Log₂ fold change (log2FC) plot of significantly (FDR-P < 0.05) enriched ASVs in responder versus non-responder mice 2 weeks post-colonization with human pooled inoculums. Filled circles located above y = 0 indicate enrichment in responders, and those below indicate enrichment in non-responders