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. 2022 Mar 28;22:151. doi: 10.1186/s12870-022-03553-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — ROS accumulation in the mnb1 mutants and MNB1-overexpressing lines. A DAB staining of Col, mnb1, OE3, OE7 lines. Arabidopsis seedlings germinated on MS agar media for 2 weeks and then shifted to MS (+Fe, control) and Fe-deficient (−Fe) for 7 days. B Quantification of H₂O₂ from Col, mnb1-1, mnb1-2, OE3 and OE7 plants. Arabidopsis seedlings germinated on MS agar media for 2 weeks and then shifted to MS (+Fe, control) and Fe-deficient (−Fe) for 7 days, and seedling samples were obtained and quantified in H₂O₂ concentration. C The measurement of MDA in Col, mnb1-1, mnb1-2, OE3 and OE7 plants. Arabidopsis seedlings germinated on MS agar plates for 2 weeks and then shifted to MS (+Fe, control) or Fe-deficient (−Fe) for 7 days, and seedling samples were obtained and quantified in MDA concentration. Values are means and SD from three to four independent biological replicates. Statistically significant differences (Tukey’s test, p < 0.05) are marked by different lowercase letters