Table 2.
Primer combinations, annealing temperatures and amplicon sizes
| Target species | Forward primer | Reverse primer | Product size (bp) | Tm (°C) |
|---|---|---|---|---|
| Wolbachia | Wspecf | Wspecr | 438 | 57 |
| Anaplasmataceae | pA (27F) | EHR 16SR | 790 | 59 |
| Anaplasmataceae | EHR 16SD | pH (1492R) | 1030 | 60 |
| Anaplasmataceae | 16S 567F | 16S 712R | 145 | 56 |
| Anaplasmataceae | 16S 567F | 16S 1401R | 834 | 56 |
| Anaplasmataceae | 16S 712F | 16S 1401R | 689 | 57 |
| Bacteria | pA (27F) | pH (1492R) | 1465 | 58 |
| Wolbachia | Wsp 81 F | Wsp 691 R | 610 | 55 |
| Wolbachia | ftsZf1 | ftsZr1 | 1043–1055 | 55 |
| S. noctilio | SnW1f | SnW1r | 420 | 52 |
| S. noctilio | SnW2f | SnW2r | 210 | 56 |
| S. noctilio | SnW3f | SnW3r | 200 | 55 |