Table 3.
Antibodies used for immunohistochemistry (IHC) and western blot (WB)
| Antibody | Host | Dilution IHC | Dilution WB | Dilution Flow cytometry | Lot number, manufacturer |
|---|---|---|---|---|---|
| GFAP | Rabbit | 1:400 | 1:1000 | – | #7260, Abcam |
| GFAP | Rat | 1:200 | – | 1:100 | #ab279291, Abcam |
| AQP4 | Rabbit | 1:1500 | 1:1000 | 1:100 | #PAB 20767, Abnova |
| S100ß | Rabbit | 1:100 | – | – | #Ab52642, Abcam |
| Transthyretin | Rabbit | – | 1:1000 | – | #A0002, Dako |
| Vimentin | Rabbit | 1:200 | – | – | #ab92547, Abcam |
| Vimentin | Mouse | 1:200 | – | – | #ab8978, Abcam |
| Anti-mouse Alexa Fluor 555 | Goat | 1:300 | – | – | #AQ21422, Thermo Fisher Scientific |
| Anti-rabbit Alexa Fluor 488 | Goat | 1:300 | – | – | #A11034, Thermo Fisher Scientific |
| Anti-rat FITC | Goat | – | – | 1:100 | #Ab6840, Abcam |
| Anti-rabbit APC | Goat | – | – | 1:100 | #Ab130805, Abcam |
| Peroxidase-conjugated anti-rabbit | – | 1:10,000 | – | #70742, Cell Signaling Technology |