TABLE 2.
Effect of treatment of mice with hM-CSF and AMPH-B on in vitro phagocytic and fungicidal activities of macrophagesa
| Treatment | % Phagocytic activity | % Fungicidal activity |
|---|---|---|
| Vehicle | 17.1 ± 1.8 | 24.6 ± 12.6 |
| hM-CSF (4 × 106 U/kg) | 21.2 ± 1.4 | 40.2 ± 16.8 |
| AMPH-B (100 μg/kg) | 19.8 ± 3.1 | 29.8 ± 22.1 |
| hM-CSF + AMPH-B | 29.5 ± 2.7b | 53.0 ± 14.9b |
a
Macrophages were prepared as described in footnote a of Table 1. After incubating macrophages and C. albicans (E/T, 2:1) for 30 min, macrophages were washed and lysed or were cultured for a further 5 h before lysis. Phagocytosis of macrophages was determined after 30 min of incubation, and fungicidal activity was determined after 5 h. Data are expressed as means ± standard deviations for triplicate samples from four experiments.
b
P < 0.05 (significant difference among the treatments).