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. 2022 Mar 28;23:86. doi: 10.1186/s13059-022-02647-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Functional characterization of BnaTT8 as a positive regulator of seed lignin biosynthesis in B. napus. a Seeds of CRISPR/Cas9-induced BnaA09.TT8 and BnaC09.TT8 homozygous double mutant lines (L14, L17, L21, L18, L24, and L30). Bar = 1 cm. b the seed coats of L21 and L24. Bar = 1 cm. Determination of thickness of seed coat (c), SCC (d), lignin content (e), and seed oil content (f) in the BnaTT8 double knockout mutant seeds and the control (WT). Values are means ± SD of three biological replicates (n = 6). Student’s t-test was used for statistical analysis between the BnaTT8-sgRNA lines and WT (*, P < 0.05)