Fig. 1.

EDA alone is not sufficient for reprogramming HDFs into iSGCs. a Flow cytometry quantification of GFP⁺ cells. Fluorescence intensity of GFP⁺ cells enriched by puromycin. Human dermal fibroblasts were transfected with EDA on day 15. b Confirmation of successful transfection with EDA by qPCR analysis. c Phase contrast images showing the morphological difference between HDF, HDF-EDA and pSGCs. Scale bar = 200 μm. Illustrations, higher magnification of the boxed areas. d qPCR analysis of transcriptional expression of CK5, CK10 and CK18 in HDF, HDF-EDA and pSGC. The genes showing significant differences in qPCR array assay were presented. n = 3. Data were expressed as mean ± SD and analyzed by two-tailed t-tests, ^*P < 0.05, ^***P < 0.001, ^****P < 0.0001, ns not significant, EDA ectodermal dysplasia antigen, HDF human dermal fibroblasts, CK5 cytokeratin 5, CK10 cytokeratin 10, CK18 cytokeratin 18, pSGC primary sweat gland cell, iSGC induced sweat gland-like cell