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. 2022 Feb 2;10(2):359. doi: 10.3390/biomedicines10020359

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Markers for M1 and M2 macrophages and cytokine expression in the aneurysm wall after 28 days on vehicle or CAG treatment. (A) Representative micrographs of CD206 positive staining from vehicle and CAG AAAs at day 28 (enlargement represent black square on the left image). Arrows mark positive cells (n = 8/7). Scale bar (black box) in micrographs to the left: 100 µm and in micrographs to the right: 50 μm. (B) Semi-quantification of CD206 positive cells in each group per mm² in adventitia. (C) Relative mRNA levels of IL-10 (n = 10/10), (D) inducible NO synthase (iNOS) (n = 10/10), and (E) IL-6 (n = 9/9) in the aneurysm tissue. All RNA data is normalized to RPL41 mRNA levels. All values are median ± interquartile range.