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ALE inhibits KLK5 protease activity. (A) KLK5 activity was measured in relative fluorescence units (RFUs) using Boc-V-P-R-AMC, fluorogenic peptide substrate. (B) KLK5 expression was measured in HEKn induced by VD3. The results are expressed as mean ± standard deviation (SD) (n = 3). * p < 0.01 vs. vehicle control; ** p < 0.05 vs. rhKLK5-treated control; § p < 0.01 vs. VD3-untreated control; *** p < 0.05 vs. VD3-treated control.
