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. 2022 Feb 16;10(2):463. doi: 10.3390/biomedicines10020463

Figure 2.

Figure 2

Bioassay-guided isolation and identification of bioactive compounds from A. lavandulaefolia inhibiting KLK5 activity. (A) Schematic representation of bioassay-guided isolation of bioactive compounds. (B) HPLC chromatogram of fractionation 3. (C) Negative ion MS/MS spectra for compounds 1 and 2. (D) The inhibitory effect of compounds 1 and 2 on KLK5 activity was measured using Boc-V-P-R-AMC, fluorogenic peptide substrate. The results are expressed as mean ± standard deviation (SD) (n = 3). * p < 0.01 vs. vehicle control; ** p < 0.01 vs. rhKLK5-treated control. ICA A, isochlorogenic acid A; ICA C, isochlorogenic acid C.