. 2022 Feb 16;10(2):463. doi: 10.3390/biomedicines10020463

Table 1.

Primer sequence for PCR.

Gene	Primer Sequence (5′-3′)	Product Size (bp)
Cma1	Forward TCTGCTGTGTGCTGGGATAG	190
Cma1	Reverse GGCACACAAAACCTGCACTA	190
Tpsab1	Forward GTGCTGGGAATGAAGGACAT	187
Tpsab1	Reverse TTGGGGACATAGTGGTGGAT	187
ICAM1	Forward GGCTGGAGCTGTTTGAGAAC	202
ICAM1	Reverse ACTGTGGGGTTCAACCTCTG	202
VCAM1	Forward CAGACAGGAAGTCCCTGGAA	212
VCAM1	Reverse TTCTTGCAGCTTTGTGGATG	212
FPR2	Forward CAACCCCATGCTTTACGTCT	184
FPR2	Reverse ATATCCCTGACCCCATCCTC	184
Mrgprx2	Forward TGAAAGCAACCATACTGGAATGTC	113
Mrgprx2	Reverse ACCACAGCACTGTGGCATTTCC	113
GAPDH	Forward TGCACCACCAACTGCTTAGC	87
GAPDH	Reverse GGCATGGACTGTGGTCATGAG	87
Gapdh	Forward CATCACTGCCACCCAGAAGACTG	153
Gapdh	Reverse ATGCCAGTGAGCTTCCCGTTCAG	153

Note: Cma1, mouse chymase; Tpsab1, mouse tryptase; ICAM1, human intercellular adhesion molecule 1; VCAM1, human vascular cell adhesion molecule 1; FPR2, human formyl peptide receptor 2; Mrgprx2, mouse MAS-related G protein-coupled receptor member X2; GAPDH, human glyceraldehyde-3-phosphate dehydrogenase; Gapdh, mouse glyceraldehyde-3-phosphate dehydrogenase.