Figure 5.

Idebenone reduces mitochondrial ROS production in ARPE-19 cells treated with H₂O₂. Idebenone pre-treatment (24 h) blocks H₂O₂-induced mitochondrial ROS accumulation. Mitochondrial ROS levels are quantified by measurement of DCF fluorescence (see Materials and Methods) and the results are expressed as a percentage of the untreated control (control = 100%). Results are from two independent experiments, the means ± SEM of 10 replicates per group. *** p < 0.001 vs. control; °°° p < 0.001 vs. H₂O₂ alone.