. 2022 Feb 7;10(2):393. doi: 10.3390/biomedicines10020393

Table 4.

Modification of some biochemical activities of cardiac sarcoplasmic reticulum, myofibrils and mitochondria upon incubation for 30 min with some oxyradical generating systems.

Parameters	Control	X+XO	1 mM H₂O₂
A. Sarcoplasmic reticulum:
Ca²⁺-release (nmol Ca²⁺/mg/15 s)	8.5 ± 1.4	4.2 ± 0.8 *	3.9 ± 0.7 *
Ca²⁺-uptake (nmol Ca²⁺/mg/min)	29.6 ± 2.4	15.9 ± 1.6 *	12.7 ± 1.5 *
Ca²⁺-pump ATPase (µmol Pi/mg/h)	14.7 ± 1.3	6.4 ± 0.8 *	5.7 ± 0.9 *
B. Myofibrils:
Mg²⁺-ATPase (µmol/mg/h)	2.53 ± 0.13	4.97 ± 0.16 *	5.46 ± 0.18 *
Ca²⁺-stimulated ATPase (µmol/mg/h)	10.29 ± 0.17	6.48 ± 0.18 *	5.92 ± 0.38 *
Sulfhydryl group content (nmol/mg protein)	67.0 ± 1.3	54.2 ± 1.6 *	47.6 ± 2.06 *
C. Mitochondria
State 3 respiration (O/mg/min)	293 ± 7.0	138 ± 7.0 *	106 ± 4.0 *
RCI (State 3 to state 4 ratio)	5.36 ± 0.13	2.66 ± 0.23 *	1.89 ± 0.07 *
ADP to O ratio (nmol ADP/ng atom O)	3.00 ± 0.15	2.55 ± 0.07 *	2.37 ± 0.03 *

The data are taken from our papers (Matsubara and Dhalla [123], Takeda et al. [124], Suzuki et al. [125] and Makazan et al. [126]). X + XO, 2 mM xanthine plus 0.03 U xanthine oxidase. This mixture was used to generate superoxide radicals. * p < 0.05 vs. respective control.